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Product Info Summary
| SKU: | M00279 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-NF2 / Merlin Monoclonal Antibody
SKU/Catalog Number
M00279
BM5222 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NF2 / Merlin Monoclonal Antibody catalog # M00279. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF2 / Merlin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00279)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACBB-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF2 / Merlin Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis.
Reactive Species
M00279 is reactive to NF2 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
69.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00279 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human THP-1 whole cell, rat PC-12 whole cell, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of NF2 using anti-NF2 antibody (M00279).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2 antigen affinity purified monoclonal antibody (M00279) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF2 at approximately 70 kDa. The expected band size for NF2 is at 70 kDa.
Specific Publications For Anti-NF2 / Merlin Monoclonal Antibody (M00279)
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5 Customer Q&As for Anti-NF2 / Merlin Monoclonal Antibody
Question
I was wanting to use your anti-NF2 / Merlin Monoclonal antibody for WB for rat smooth muscle tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat smooth muscle tissue identification?
A. Jones
Verified customer
Asked: 2020-05-05
Answer
You can see on the product datasheet, M00279 anti-NF2 / Merlin Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat smooth muscle tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for smooth muscle tissue using anti-NF2 / Merlin Monoclonal antibody M00279. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-25
Question
Is a blocking peptide available for product anti-NF2 / Merlin Monoclonal antibody (M00279)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We do provide the blocking peptide for product anti-NF2 / Merlin Monoclonal antibody (M00279). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-24
Question
We want to test anti-NF2 / Merlin Monoclonal antibody M00279 on rat smooth muscle tissue for research purposes, then I may be interested in using anti-NF2 / Merlin Monoclonal antibody M00279 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Miller
Verified customer
Asked: 2019-10-03
Answer
The products we sell, including anti-NF2 / Merlin Monoclonal antibody M00279, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-NF2 / Merlin Monoclonal antibody M00279 work on monkey WB with cervix carcinoma?
C. Jackson
Verified customer
Asked: 2016-01-21
Answer
Our lab technicians have not validated anti-NF2 / Merlin Monoclonal antibody M00279 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NF2 / Merlin Monoclonal antibody M00279 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-21
