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- Table of Contents
Merlin Antibodies
AND NF2 ELISA kits, Merlin Proteins
Many biological assays use antibodies to detect Merlin, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Merlin in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Merlin antibodies...
Designing a Western blot for Merlin? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NF2 Western Blot GuideDesigning an IHC experiment for Merlin? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the NF2 IHC GuideNF2 Infographic by Boster Bio
All NF2 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00279-2
100 μg/vial
Cat # A00279-2
100 μg/vial
Human, Mouse, Rat
ELISA
$540
Cat # EKC2460
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2460
1 kit, containing two 96-well plates and all necessary reagents
Human, Mouse, Rat
ELISA
$480
Cat # EKC1363
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1363
1 kit, containing one 96-well plate and all necessary reagents
NF2 Overview
Facts about Merlin.
Merlin (NF2)
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway.
May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating action.
Gene Information
| Human | |
|---|---|
| Gene Name: | NF2 |
| Uniprot: | P35240 |
| Entrez: | 4771 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
ACN; BANF; Merlin; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); neurofibromin-2; NF2; SCH; Schwannomerlin; Schwannomin
Molecular Weight
Mass (kDA):
69.69 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 22q12.2 |
| Sequence: | 22; NC_000022.11 (29603556..29698600) |
Tissue Specificity
Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.
Subcellular Localizations
[Isoform 1]: Cell projection, filopodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. In a fibroblastic cell line, isoform 1 is found homogeneously distributed over the entire cell, with a particularly strong staining in ruffling membranes and filopodia. Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predomi
Diseases
- Neurofibromatosis 2
- Neoplasms
- Neurofibromatoses
- Neurilemmoma
- Meningioma
- Acoustic Neuroma
- Cell Transformation, Neoplastic
- Neurofibromatosis 1
- Malignant Paraganglionic Neoplasm
- Malignant Neoplasms
- Nervousness
- Meningeal Neoplasms
- Brain Neoplasms
Interacting Proteins
- AMOT
- GTPBP4
- HGS
- LATS1
- MAP3K11
- MED28
- PATJ
- Ppp1r12a
- Schip1
- SLC9A3R1
Pathways
- Pathogenesis
- Cell Proliferation
- Cell Growth
- Localization
- Contact Inhibition
- Methylation
- Cell Adhesion
- Cell Cycle
- Oncogenesis
- Cell Motility
- Schwann Cell Proliferation
- Regulation Of Cell Proliferation
PTMs
- Phosphorylation
- Methylation
- Cleavage
- Dephosphorylation
Research Areas
- Cancer
- Cell Cycle and Replication
- Tumor Suppressors
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NF2
bosterbio.com/bosterbio-gene-info-cards/NF2
References
PMID: 8453669 by Trofatter J.A., et al. A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor.
PMID: 8379998 by Rouleau G.A., et al. Alteration in a new gene encoding a putative membrane-organizing protein causes neuro-fibromatosis type 2.