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Product Info Summary
| SKU: | M00969 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NFAT1 NFATC2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00969
BM5047 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NFAT1 NFATC2 Rabbit Monoclonal Antibody catalog # M00969. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFAT1 NFATC2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00969)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAFG-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NFAT1
Reactive Species
M00969 is reactive to NFATC2 in Human
Observed Molecular Weight
135 kDa
Calculated molecular weight
100.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00969 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of NFAT1 expression in Ramos cell lysate.
Specific Publications For Anti-NFAT1 NFATC2 Rabbit Monoclonal Antibody (M00969)
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Customer Q&As
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5 Customer Q&As for Anti-NFAT1 NFATC2 Rabbit Monoclonal Antibody
Question
Will anti-NFAT1 Rabbit Monoclonal antibody M00969 work on primate WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Our lab technicians have not validated anti-NFAT1 Rabbit Monoclonal antibody M00969 on primate. You can run a BLAST between primate and the immunogen sequence of anti-NFAT1 Rabbit Monoclonal antibody M00969 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-28
Question
Is there a BSA free version of anti-NFAT1 Rabbit Monoclonal antibody M00969 available?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NFAT1 Rabbit Monoclonal antibody M00969 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-21
Question
Is this M00969 anti-NFAT1 Rabbit Monoclonal antibody reactive to the isotypes of NFATC2?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The immunogen of M00969 anti-NFAT1 Rabbit Monoclonal antibody is A synthesized peptide derived from human NFAT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-17
Question
I see that the anti-NFAT1 Rabbit Monoclonal antibody M00969 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-08
Question
Does anti-NFAT1 Rabbit Monoclonal antibody M00969 work for WB with thoracic mammary gland?
D. Jha
Verified customer
Asked: 2015-04-02
Answer
According to the expression profile of thoracic mammary gland, NFATC2 is highly expressed in thoracic mammary gland. So, it is likely that anti-NFAT1 Rabbit Monoclonal antibody M00969 will work for WB with thoracic mammary gland.
Boster Scientific Support
Answered: 2015-04-02
