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Product Info Summary
| SKU: | M00340 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00340
BM5123 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody catalog # M00340. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00340)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABDA-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NFAT2
Reactive Species
M00340 is reactive to NFATC1 in Human
Observed Molecular Weight
101 kDa
Calculated molecular weight
101.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00340 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
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Western blot analysis of NFAT2 expression in Ramos cell lysate.
Specific Publications For Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody (M00340)
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Customer Q&As
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4 Customer Q&As for Anti-NFAT2 NFATC1 Rabbit Monoclonal Antibody
Question
We are currently using anti-NFAT2 Rabbit Monoclonal antibody M00340 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-NFAT2 Rabbit Monoclonal antibody (M00340) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
We bought anti-NFAT2 Rabbit Monoclonal antibody for WB on peripheral blood lymphocyte t-cell in a previous project. I am using human, and We intend to use the antibody for IF next. I would like examining peripheral blood lymphocyte t-cell as well as b-cell lymphoma in our next experiment. Could give a recommendation on which antibody would work the best for IF?
K. Lewis
Verified customer
Asked: 2019-06-11
Answer
I looked at the website and datasheets of our anti-NFAT2 Rabbit Monoclonal antibody and it appears that M00340 has been validated on human in both WB and IF. Thus M00340 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-11
Question
We have seen staining in human b-cell lymphoma. Are there any suggestions? Is anti-NFAT2 Rabbit Monoclonal antibody supposed to stain b-cell lymphoma positively?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
According to literature b-cell lymphoma does express NFATC1. According to Uniprot.org, NFATC1 is expressed in muscle organ, peripheral blood lymphocyte t-cell, b-cell, b-cell lymphoma, brain, erythroleukemia, among other tissues. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2019-05-16
Question
My team were happy with the WB result of your anti-NFAT2 Rabbit Monoclonal antibody. However we have seen positive staining in erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is NFATC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
From what I have seen in literature, erythroleukemia does express NFATC1. Generally NFATC1 expresses in cytoplasm. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141
Boster Scientific Support
Answered: 2018-11-15
