Product Info Summary
SKU: | A03394-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-NG2 CSPG4 Antibody
SKU/Catalog Number
A03394-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NG2 CSPG4 Antibody catalog # A03394-1. Tested in WB,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NG2 CSPG4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03394-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Keratin 16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03394-1 is reactive to CSPG4 in Human
Applications
A03394-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
250.537kDa
Background of NG2/MCSP
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.
Qian Wang, J. Virol., Jan 2004; 78: 821 - 833. RD Paladini,
RD Paladini, J. Cell Biol., Feb 1996; 132: 381.
M.K. Shamsher, Hum. Mol. Genet., Oct 1995; 4: 1875 - 1881.
Ying-Nai Wang, Mol. Pharmacol., Jan 2006; 69: 85 - 98.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
FC: 1:50-1:100
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of CSPG4 using Anti-NG2 CSPG4 Antibody (A03394-1).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 2. Immunohistochemistry validation of CSPG4 using Anti-NG2 CSPG4 Antibody (A03394-1).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NG2 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For CSPG4 (Source: Uniprot.org, NCBI)
Gene Name
CSPG4
Full Name
Chondroitin sulfate proteoglycan 4
Weight
250.537kDa
Alternative Names
AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8 CSPG4 CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2 chondroitin sulfate proteoglycan 4 chondroitin sulfate proteoglycan 4|chondroitin sulfate proteoglycan 4 (melanoma-associated)|chondroitin sulfate proteoglycan NG2|melanoma chondroitin sulfate proteoglycan|melanoma-associated chondroitin sulfate proteoglycan
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSPG4, check out the CSPG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSPG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NG2 CSPG4 Antibody (A03394-1)
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No publications found for A03394-1
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