Anti-Niban FAM129A Antibody

Product Name

Anti-Niban FAM129A Antibody

View all NIBAN1 Antibodies

SKU/Catalog Number

A07791

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Niban FAM129A Antibody (Catalog # A07791). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Niban FAM129A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07791)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Clone Number

1A3

Isotype

IgG

Immunogen

peptide sequence around aa. 912~916 (K-E-G-E-G) derived from Human Niban.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07791 is reactive to NIBAN1 in Human

Applications

A07791 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

103.135kDa

Background of NIBAN1

Regulates phosphorylation of a number of proteins involved in translation regulation including EIF2A, EIF4EBP1 and RPS6KB1. May be involved in the endoplasmic reticulum stress response

Vill

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 150kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of FAM129A using anti-FAM129A antibody (A07791).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129A antigen affinity purified polyclonal antibody (Catalog # A07791) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FAM129A.

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Immunofluorescence staining of methanol-fixed Hela cells using Niban Antibody #A07791.

Gene/Protein Information For NIBAN1 (Source: Uniprot.org, NCBI)

Alternative Names

Protein Niban 1 NIBAN1 C1orf24, FAM129A, GIG39, NIBAN niban apoptosis regulator 1 protein Niban 1|cell growth-inhibiting gene 39 protein|family with sequence similarity 129 member A

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