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- Table of Contents
NCLN antibody (A12284-1) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.
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Anti-Nicalin NCLN Antibody
NCLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Nicalin NCLN Antibody
View all NCLN Antibodies
View all NCLN Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
NCLN antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
NCLN Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN. The immunogen is located within amino acids 390 - 440 of NCLN.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
63.0 kDa
Background of NCLN
Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Cite This Product
Anti-Nicalin NCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12284-1)
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Product Promise
We promise Anti-Nicalin NCLN Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A12284-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 20, 2026