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Product Info Summary
| SKU: | A12284-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Nicalin NCLN Antibody
SKU/Catalog Number
A12284-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Nicalin NCLN Antibody (Catalog # A12284-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
NCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Nicalin NCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12284-1)
Host
Rabbit
Contents
NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN. The immunogen is located within amino acids 390 - 440 of NCLN.
Reactive Species
A12284-1 is reactive to NCLN in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
63.0 kDa
Background of NCLN
Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12284-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.
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Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.
Specific Publications For Anti-Nicalin NCLN Antibody (A12284-1)
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