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- Table of Contents
CHRNA1 antibody (PA1203) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody, PA1203, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
WB: Rat Skeletal Muscle Tissue Lysate
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Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband®
CHRNA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
CHRNA1 antibody was positively detected by WB in:
Rat Skeletal Muscle Tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Human
Predicted
Monkey
Predicted
Mouse
Predicted
Rabbit
Predicted
Product Information
Product Name
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband®
View all CHRNA1 Antibodies
View all CHRNA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Nicotinic Acetylcholine Receptor alpha 1, identical to the related rat sequence, different from the related mouse sequence by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
69 kDa
Calculated Molecular Weight
54.5 kDa
Background of CHRNA1
CHRNA, also termed ACHRA, is mapped on 2q24-q32.This gene encodes the alpha subunit of the muscle acetylcholine receptor, which is the main target of pathogenic autoantibodies in autoimmune myasthenia gravis. The protein-coding sequence of the human alpha subu gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule.
Cite This Product
Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1203)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rat
100%
High Homology
Monkey
100%
High Homology
Mouse
93%
High Homology
Rabbit
93%
High Homology
Cow
87%
Moderate Homology
Dog
87%
Moderate Homology
Sheep
73%
Moderate Homology
Zebrafish
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CHRNA1 antibody
Publications
Application:
Species:
Su Y,Ahn B,Macpherson PCD,Ranjit R,Claflin DR,Van Remmen H,Brooks SV.Transgenic expression of SOD1 specifically in neurons of Sod1 deficient mice prevents defects in muscle mitochondrial function and calcium handling.Free Radic Biol Med.2021 Feb 6:S0891-5
Species: Mouse
PMID: 33561489
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Product Promise
We promise Anti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1203
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026