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Product Info Summary
| SKU: | A07135 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-NIPA ZC3HC1 Antibody
SKU/Catalog Number
A07135
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NIPA ZC3HC1 Antibody catalog # A07135. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NIPA ZC3HC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07135)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NIPA. AA range:320-369
Reactive Species
A07135 is reactive to ZC3HC1 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07135 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using NIPA Polyclonal antibody.
Specific Publications For Anti-NIPA ZC3HC1 Antibody (A07135)
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Customer Q&As
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7 Customer Q&As for Anti-NIPA ZC3HC1 Antibody
Question
I have a question about product A07135, anti-NIPA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07135 anti-NIPA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this A07135 anti-NIPA antibody reactive to the isotypes of ZC3HC1?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
The immunogen of A07135 anti-NIPA antibody is Synthesized peptide derived from human NIPA around the non-phosphorylation site of S354. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-29
Question
I was wanting to use your anti-NIPA antibody for WB for monkey mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for monkey mammary gland identification?
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
As indicated on the product datasheet, A07135 anti-NIPA antibody has been tested for WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in monkey mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-18
Question
Would A07135 anti-NIPA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-13
Answer
It shows on the product datasheet, A07135 anti-NIPA antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-13
Question
We are currently using anti-NIPA antibody A07135 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
C. Jones
Verified customer
Asked: 2016-08-11
Answer
The anti-NIPA antibody (A07135) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-11
Question
I see that the anti-NIPA antibody A07135 works with WB, what is the protocol used to produce the result images on the product page?
K. Mitchell
Verified customer
Asked: 2013-12-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-12-17
Question
We are interested in to test anti-NIPA antibody A07135 on monkey mammary gland for research purposes, then I may be interested in using anti-NIPA antibody A07135 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Wu
Verified customer
Asked: 2013-08-19
Answer
The products we sell, including anti-NIPA antibody A07135, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-08-19
