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NOS2 antibody (A00368) performs well in IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 16 Publications

a00368 nos2 primary antibodies ihc testing 1

IHC analysis of iNOS/NOS2 using anti-iNOS/NOS2 antibody (A00368).
iNOS/NOS2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-iNOS/NOS2 Antibody (A00368) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-iNOS/NOS2 Antibody Picoband®

NOS2 antibody

16 Publications

Reactive Species	Host	Application
Human	Rabbit	IHC, WB

Tested Applications

NOS2 antibody was positively detected by WB in:

human Hela whole cell, human Caco-2 whole cell, human SH-SY5Y whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

NOS2 antibody was positively detected by IHC in:

human brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Dog

Predicted

Rat

Predicted

Product Information

Product Name

Anti-iNOS/NOS2 Antibody Picoband®
View all NOS2 Antibodies

Form

Lyophilized

Uniprot

P35228

Gene ID

4843

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human iNOS, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

131 kDa

Calculated Molecular Weight

131.1 kDa

Background of NOS2

Nitric oxide synthase, inducible is an enzyme that in humans is encoded by the NOS2 gene. Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. Three different NOS isoforms have been identified which fall into two distinct types, constitutive and inducible. The inducible NOS (iNOS) isoform is expressed in a variety of cell types and tissues in response to inflammatory agents and cytokines. The human iNOS (NOS2) gene is approximately 37 kb in length and consists of 26 exons and 25 introns. NOS2-derived NO is a prerequisite for cytokine signaling and function in innate immunity.

Cite This Product

Anti-iNOS/NOS2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00368)

Protocols

Western Blot protocol for NOS2 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for NOS2 antibody

Review Protocol

Publications

miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis

Published: 2026-05-04

Journal: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Application: WB

Impact Factor: 3.50

Species: Human

PMID: 42092414

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Anti-iNOS/NOS2 Antibody Picoband®

NOS2 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

miR-33a promotes peri-implantitis by facilitating M1-type polarization of macrophages and inhibiting osteogenesis

Tea polyphenols ameliorates insulin resistance and inflammation by modulating macrophage polarization via JAK2/STAT3 pathway in aged T2DM combined with NAFLD rats

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Distinct roles of TREM2 in central nervous system cancers and peripheral cancers

All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells

Diosgenin Attenuates Lipopolysaccharide-Induced Parkinson’s Disease by Inhibiting the TLR/NF- κB Pathway

Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model

Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the L-NAME-induced hypertensive rat model

The role of NF-κB in PARP-inhibitor-mediated sensitization and detoxification of arsenic trioxide in hepatocellular carcinoma cells

HIF‑1 signaling pathway involving iNOS, COX‑2 and caspase‑9 mediates the neuroprotection provided by erythropoietin in the retina of chronic ocular hypertension rats

Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury

Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats

2,3,4′,5-Tetrahydroxystilbene-2-O-β-d-glucoside Inhibits Proliferation of Vascular Smooth Muscle Cells: Involvement of NO/cGMP/PKG Pathway

Sildenafil improves diabetic vascular activity through suppressing endothelin receptor A, iNOS and NADPH oxidase which is comparable with the endothelin receptor antagonist CPU0213 in STZ-injected rats

Effect of erythropoietin on the expression of HIF-1 and iNOS in retina in chronic ocular hypertension rats

Review

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