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Product Info Summary
| SKU: | A30848 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NK3R Antibody
SKU/Catalog Number
A30848
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NK3R Antibody (Catalog# A30848). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NK3R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30848)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NK3R. Immunogen sequence location: 401 - 450
Reactive Species
A30848 is reactive to TACR3 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
52.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30848 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of COLO205 cells using NK-3R Polyclonal Antibody.
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Western Blot analysis of various cells using NK-3R Polyclonal Antibody.
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Western blot analysis of the lysates from HepG2 cells using NK3R antibody.
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Immunofluorescence analysis of A549 cells, using NK3R Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO205 cells, using NK3R Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-NK3R Antibody (A30848)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-NK3R Antibody
Question
Is a blocking peptide available for product anti-NK3R antibody (A30848)?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do provide the blocking peptide for product anti-NK3R antibody (A30848). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-13
Question
We are currently using anti-NK3R antibody A30848 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The anti-NK3R antibody (A30848) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-24
Question
I am interested in to test anti-NK3R antibody A30848 on rat hypothalamus for research purposes, then I may be interested in using anti-NK3R antibody A30848 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The products we sell, including anti-NK3R antibody A30848, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-20
Question
Will anti-NK3R antibody A30848 work on monkey IF with brain?
D. Edwards
Verified customer
Asked: 2019-12-16
Answer
Our lab technicians have not validated anti-NK3R antibody A30848 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NK3R antibody A30848 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-16
Question
Would anti-NK3R antibody A30848 work for WB with hypothalamus?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
According to the expression profile of hypothalamus, TACR3 is highly expressed in hypothalamus. So, it is likely that anti-NK3R antibody A30848 will work for WB with hypothalamus.
Boster Scientific Support
Answered: 2019-11-15
Question
I was wanting to use your anti-NK3R antibody for WB for rat hypothalamus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat hypothalamus identification?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can see on the product datasheet, A30848 anti-NK3R antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat hypothalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-30
Question
Is this A30848 anti-NK3R antibody reactive to the isotypes of TACR3?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The immunogen of A30848 anti-NK3R antibody is A peptide derived from human NK3R. Immunogen sequence location: 401 - 450. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-01
