Anti-NK3R TACR3 Antibody

Product Name

Anti-NK3R TACR3 Antibody

View all TACR3 Antibodies

SKU/Catalog Number

A04916

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NK3R TACR3 Antibody (Catalog # A04916). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

NK3R antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NK3R TACR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04916)

Host

Rabbit

Contents

NK3R Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R. The immunogen is located within amino acids 370 - 420 of NK3R.

Cross-reactivity

NK3R antibody does not recognize NK1R or NK2R.

Reactive Species

A04916 is reactive to TACR3 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

52.2 kDa

Background of TACR3

The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04916 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TACR3

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