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Product Info Summary
| SKU: | A09210-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-NKX2-3 Antibody
SKU/Catalog Number
A09210-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NKX2-3 Antibody catalog # A09210-1. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NKX2-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09210-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NKX2-3. Human NKX2-3 shares 75% amino acid (aa) sequence identity with mouse NKX2-3.
Reactive Species
A09210-1 is reactive to NKX2-3 in Human
Calculated molecular weight
38.4 kDa
Background of NKX2-3
This gene encodes a homeodomain-containing transcription factor. The encoded protein is a member of the NKX family of homeodomain transcription factors. Studies of similar proteins in mouse and rat have indicated a potential role in cellular differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09210-1 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Validation Images & Assay Conditions
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IHC analysis of NKX2-3 using anti-NKX2-3 antibody (A09210-1).
NKX2-3 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-3 Antibody (A09210-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-NKX2-3 Antibody (A09210-1)
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