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Product Info Summary
| SKU: | A30727 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NKX26 Antibody
SKU/Catalog Number
A30727
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NKX26 Antibody (Catalog# A30727). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NKX26 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30727)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NKX26. Immunogen sequence location: 91-140
Reactive Species
A30727 is reactive to NKX2-6 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
32.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30727 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Nkx-2.6 Polyclonal Antibody diluted at 1:500
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Western blot analysis of the lysates from Jurkat cells using NKX26 antibody.
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Western blot analysis of lysates from HeLa and COLO cells, using NKX26 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-NKX26 Antibody (A30727)
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Customer Q&As
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3 Customer Q&As for Anti-NKX26 Antibody
Question
We are currently using anti-NKX26 antibody A30727 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
The anti-NKX26 antibody (A30727) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-30
Question
Can you help my question with product A30727, anti-NKX26 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Bhatt
Verified customer
Asked: 2018-08-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30727 anti-NKX26 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-07
Question
Will anti-NKX26 antibody A30727 work for WB with right atrium auricular region?
S. Parker
Verified customer
Asked: 2016-05-12
Answer
According to the expression profile of right atrium auricular region, NKX2-6 is highly expressed in right atrium auricular region. So, it is likely that anti-NKX26 antibody A30727 will work for WB with right atrium auricular region.
Boster Scientific Support
Answered: 2016-05-12
