Product Info Summary
SKU: | M02091-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-NLK Rabbit Monoclonal Antibody
SKU/Catalog Number
M02091-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-NLK Rabbit Monoclonal Antibody catalog # M02091-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NLK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02091-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
25N97
Isotype
IgG
Immunogen
A synthesized peptide derived from human NLK
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02091-1 is reactive to Nlk in Human
Applications
M02091-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
58 kDa
Calculated molecular weight
58.313kDa
Background of Nlk
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NLK using anti-NLK antibody (M02091-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLK antigen affinity purified monoclonal antibody (Catalog # M02091-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLK at approximately 58 kDa. The expected band size for NLK is at 58 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Nlk (Source: Uniprot.org, NCBI)
Gene Name
Nlk
Full Name
Serine/threonine-protein kinase NLK
Weight
58.313kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp761G1211; EC 2.7.11; EC 2.7.11.24; FLJ21033; LAK1; nemo like kinase; nemo-like kinaseserine/threonine-protein kinase NLK; Protein LAK1 Nlk|AI194375|nemo like kinase|serine/threonine-protein kinase NLK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nlk, check out the Nlk Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nlk: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NLK Rabbit Monoclonal Antibody (M02091-1)
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No publications found for M02091-1
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