Human MMP-3 PicoKine™ Quick ELISA Kit

Human MMP-3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK0461
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)
Assay Time: 90 Minutes

Product Name

Human MMP-3 PicoKine™ Quick ELISA Kit

See all MMP-3 products

SKU/Catalog Number

FEK0461

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-3 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-3 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0461)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0461 is reactive to MMP3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK0461 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0461-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0461-STHuman MMP-3 Standard 2 10 ng/tube
FEK0461-CHuman MMP3 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0420.1480.2310.3920.7161.2542.0612.405

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2881542469731517654511
Standard deviation19.0198.69295.9120.16118.26311.26
CV (%)6.6%6.4%6.3%6.4%6.7%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128830130526729018.296.3%
Sample 21542154816861602159552.623.3%
Sample 346973934451245394421256.395.8%
*number of samples for each test n=16.

Gene/Protein Information For MMP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08254

Gene ID

4314

Gene Name

MMP3

Full Name

Stromelysin-1

Weight

53.977kDa

Superfamily

peptidase M10A family

Alternative Names

CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP3, check out the MMP3 Infographic

MMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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