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- Table of Contents
Nlrp4g antibody (PA1687) performs well in IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of NLRP4G using anti-NLRP4G antibody (PA1687).
NLRP4G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NLRP4G Antibody (PA1687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NLRP4G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NLRP4G Antibody (PA1687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NLRP4G Antibody Picoband®
Nlrp4g antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | IHC, WB |
Tested Applications
Nlrp4g antibody was positively detected by WB in:
Mouse Ovary Tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Nlrp4g antibody was positively detected by IHC in:
human lung cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse
Western blot
0.1-0.5μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-NLRP4G Antibody Picoband®
View all NLRP4G Antibodies
View all NLRP4G Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NLRP4G.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Background of Nlrp4g
Stra8, stimulated by retinoic acid 8, is expressed in embryonic ovaries just before meiotic initiation, whereas in testis it is expressed after birth. The STRA8 gene contains 9 exons. And this gene is mapped to chromosome 7q31. Retinoic acid (RA) signaling was required for Stra8 expression, and therefore initiation of meiosis, in embryonic mouse ovaries. RA was sufficient to induce Stra8 expression in embryonic testis. Cyp26-mediated RA metabolism was responsible for delaying Stra8 expression in embryonic testis. Since RA signaling regulated Stra8 expression in both embryonic ovaries and adult testis, it was said this portion of the meiotic initiation pathway may be identical in both sexes.
Cite This Product
Anti-NLRP4G Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1687)
Protocols
Western Blot protocol for Nlrp4g antibody
Immunohistochemistry (IHC) protocol for Nlrp4g antibody
Publications
Application:
Expression and localization of Nlrp4g in mouse preimplantation embryo
Published: 2014-11-11
Journal: ZYGOTE
Impact Factor: 1.32
PMID: 25384339
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Product Promise
We promise Anti-NLRP4G Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1687
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026