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Product Info Summary
| SKU: | A04481 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NOD4 NLRC5 Antibody
SKU/Catalog Number
A04481
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NOD4 NLRC5 Antibody (Catalog # A04481). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
NOD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NOD4 NLRC5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04481)
Host
Rabbit
Contents
NOD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4. The immunogen is located within amino acids 20 - 70 of NOD4.
Cross-reactivity
NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
Reactive Species
A04481 is reactive to NLRC5 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
204.6 kDa
Background of NLRC5
NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κB and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04481 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NOD4 antibody can be used for detection of NOD4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of NOD4 in EL4 cell lysate with NOD4 antibody at 1 μg/mL.
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Immunocytochemistry of NOD4 in HeLa cells with NOD4 antibody at 10 μg/mL.
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Immunofluorescence of NOD4 in HeLa cells with NOD4 antibody at 20 μg/mL.
Specific Publications For Anti-NOD4 NLRC5 Antibody (A04481)
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