Anti-NADPH oxidase 3 Nox3 Antibody

NOX3 antibody

Boster Bio Anti-NADPH oxidase 3 Nox3 Antibody catalog # A05715. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05715
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC-P, WB

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Product Name

Anti-NADPH oxidase 3 Nox3 Antibody

View all NOX3 Antibodies

SKU/Catalog Number

A05715

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NADPH oxidase 3 Nox3 Antibody catalog # A05715. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADPH oxidase 3 Nox3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05715)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Nox3. at AA range: 260-340

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05715 is reactive to NOX3 in Human, Mouse, Rat

Applications

A05715 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:100-1:300
ELISA, 1:40000

Validation Images & Assay Conditions

Gene/Protein Information For NOX3 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9HBY0

Gene ID

50508

Gene Name

NOX3

Full Name

NADPH oxidase 3

Weight

64.935kDa

Alternative Names

EC 1.6.3; GP91-3EC 1.6.3.-; gp91phox homolog 3; Mitogenic oxidase 2; MOX2; MOX-2; NADPH oxidase 3; NADPH oxidase catalytic subunit-like 3 NOX3 GP91-3, MOX-2 NADPH oxidase 3 NADPH oxidase 3|NADPH oxidase catalytic subunit-like 3|gp91phox homolog 3|mitogenic oxidase 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOX3, check out the NOX3 Infographic

NOX3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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4 Customer Q&As for Anti-NADPH oxidase 3 Nox3 Antibody

Question

We are currently using anti-Nox3 antibody A05715 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

The anti-Nox3 antibody (A05715) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-28

Question

Does A05715 anti-Nox3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-11-06

Answer

As indicated on the product datasheet, A05715 anti-Nox3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-11-06

Question

Would anti-Nox3 antibody A05715 work for IHC with kidney?

E. Bhatt

Verified customer

Asked: 2018-05-17

Answer

According to the expression profile of kidney, NOX3 is highly expressed in kidney. So, it is likely that anti-Nox3 antibody A05715 will work for IHC with kidney.

Boster Scientific Support

Answered: 2018-05-17

Question

I have a question about product A05715, anti-Nox3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Dhar

Verified customer

Asked: 2014-06-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05715 anti-Nox3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-06-03

Size

Total: $399

SKU:A05715

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A05715
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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