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Product Info Summary
| SKU: | A05715 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-NADPH oxidase 3 Nox3 Antibody
SKU/Catalog Number
A05715
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NADPH oxidase 3 Nox3 Antibody catalog # A05715. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NADPH oxidase 3 Nox3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05715)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NOX3. AA range:287-336
Reactive Species
A05715 is reactive to NOX3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
64.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05715 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NOX3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using Nox3 Polyclonal Antibody
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Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-NADPH oxidase 3 Nox3 Antibody (A05715)
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Customer Q&As
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4 Customer Q&As for Anti-NADPH oxidase 3 Nox3 Antibody
Question
We are currently using anti-Nox3 antibody A05715 for rat tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
The anti-Nox3 antibody (A05715) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-28
Question
Does A05715 anti-Nox3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
As indicated on the product datasheet, A05715 anti-Nox3 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-06
Question
Would anti-Nox3 antibody A05715 work for IHC with kidney?
E. Bhatt
Verified customer
Asked: 2018-05-17
Answer
According to the expression profile of kidney, NOX3 is highly expressed in kidney. So, it is likely that anti-Nox3 antibody A05715 will work for IHC with kidney.
Boster Scientific Support
Answered: 2018-05-17
Question
I have a question about product A05715, anti-Nox3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Dhar
Verified customer
Asked: 2014-06-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05715 anti-Nox3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-03
