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- Table of Contents
NR3C1 antibody (M00503-2) performs well in WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
All lanes : Anti-NR3C1 at dilution
Lane 1: DU145 whole cell lysate
Lane 2: U-251MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: DU145 whole cell lysate
Lane 2: U-251MG whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 86 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Designing a Western blot for NR3C1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NR3C1 Western Blot GuideBundle Save
Anti-NR3C1
NR3C1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Mouse | WB |
Recommend Dilution
Application
Dilution
Species
WB: 1:2000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Product Information
Product Name
Anti-NR3C1
View all NR3C1 Antibodies
View all NR3C1 Antibodies
Uniprot
Gene ID
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2153CT307.32.80
Isotype
IgG1,κ
Immunogen
This antibody is generated from a mouse immunized with a recombinant protein from human.
Calculated Molecular Weight
85.7 kDa
Background of NR3C1
Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity).
Cite This Product
Anti-NR3C1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00503-2)
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Product Promise
We promise Anti-NR3C1 performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00503-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 30, 2026