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Product Info Summary
| SKU: | PB9342 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband®
SKU/Catalog Number
PB9342
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® catalog # PB9342. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9342)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NR3C1 recombinant protein (Position: A20-F199). Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9342 is reactive to NR3C1 in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
85.7 kDa
Background of NR3C1
The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9342 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human placenta tissue, human Hela whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
IHC: rat cardiac muscle tissue, human lung cancer tissue, mouse cardiac muscle tissue
Validation Images & Assay Conditions
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Western blot analysis of NR3C1 using anti-NR3C1 antibody (PB9342).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody (Catalog # PB9342) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 100 kDa. The expected band size for NR3C1 is at 86 kDa.
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IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342).
NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342).
NR3C1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342).
NR3C1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® (PB9342)
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2 Customer Q&As for Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband®
Question
What cell lysis protocol was used for the cell samples of PB9342?
Verified customer
Asked: 2019-12-17
Answer
For the Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband™ (PB9342), please see the following cell lysis protocol. 1. Rinse cells in ice-cold PBS. 2. Scrape cells or trypsinize cells and wash with PBS. 3. Centrifuge at ~3000 x g for 2-3 minutes, remove PBS. The centrifugation force and time can be varied depending on the cell type. 4. Add lysis buffer. 5. Sonicate for 10–15 sec to complete cell lysis. 6. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the cell type. 7. Aspirate supernatant avoiding pellet and upper, lipid-containing phase. 8. Aliquot supernatant and store at -20°C.
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-Glucocorticoid Receptor/NR3C1 antibody PB9342 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
L. Banerjee
Verified customer
Asked: 2019-10-16
Answer
The anti-Glucocorticoid Receptor/NR3C1 antibody (PB9342) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
