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- Table of Contents
NR3C1 antibody (PB9342) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342).
NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband®
NR3C1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
NR3C1 antibody was positively detected by WB in:
human placenta tissue, human Hela whole cell, human A549- WT whole cell, human A549-NR3C1 KO whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NR3C1 antibody was positively detected by IHC in:
rat cardiac muscle tissue, human lung cancer tissue, mouse cardiac muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband®
View all NR3C1 Antibodies
View all NR3C1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NR3C1 recombinant protein (Position: A20-F199). Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
85.7 kDa
Background of NR3C1
The glucocorticoid receptor (GR, or GCR), also known as NR3C1, is the receptor to which cortisol and other glucocorticoids bind. In humans, the GR protein is encoded by NR3C1 gene which is located on chromosome 5 (5q31). GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. Because the receptor gene is expressed in several forms, it has many different (pleiotropic) effects in different parts of the body. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol (by preventing the translocation of other transcription factors from the cytosol into the nucleus).
Cite This Product
Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9342)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Cow
93%
High Homology
Dog
91%
High Homology
Sheep
90%
High Homology
Mouse
82%
Moderate Homology
Rat
82%
Moderate Homology
Rabbit
76%
Moderate Homology
Zebrafish
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NR3C1 antibody
Immunohistochemistry (IHC) protocol for NR3C1 antibody
Publications
Application:
Species:
PERK inhibitor (ISRIB) improves depression-like behavior by inhibitions of HPA-axis over-activation in mice exposed to chronic restraint stress
Published: 2024-06-26
Journal: BEHAVIOURAL BRAIN RESEARCH
Application: IHC
Impact Factor: 2.60
Species: Mouse
PMID: 38942086
Effect of rapid eye movement sleep deprivation during pregnancy on glucocorticoid receptor regulation of HPA axis function in female offspring
Published: 2022-02-10
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 3.61
Species: Rat
PMID: 35151654
Role of p300 in the pathogenesis of Henoch-Schonlein purpura nephritis and as a new target of glucocorticoid therapy in mice
Published: 2019-08-20
Journal: CHINESE MEDICAL JOURNAL
Application: WB
Impact Factor: 1.56
Species: Mouse
PMID: 31365430
Endocannabinoid hydrolase and cannabinoid receptor 1 are involved in the regulation of hypothalamus-pituitary-adrenal axis in type 2 diabetes
Published: 2018-06-11
Journal: METABOLIC BRAIN DISEASE
Application: IHC
Impact Factor: 2.44
Species: Monkey
PMID: 29948652
Effects of group housing on stress induced emotional and neuroendocrine alterations
Published: 2013-02-01
Journal: BRAIN RESEARCH
Application: IHC
Impact Factor: 2.88
Species: Mouse
PMID: 23380532
Expression of locus coeruleus mineralocorticoid receptor and glucocorticoid receptor in rats under single-prolonged stress
Published: 2011-05-17
Journal: NEUROLOGICAL SCIENCES
Application: IHC,WB
Impact Factor: 1.22
Species: Rat
PMID: 21584742
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Product Promise
We promise Anti-Glucocorticoid Receptor/NR3C1 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9342
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026