Anti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband™

Product Name

Anti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband™

View all Mineralocorticoid R/NR3C2 Antibodies

SKU/Catalog Number

PB9765

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband™ catalog # PB9765. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9765)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human NR3C2, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9765 is reactive to NR3C2 in Human, Mouse, Rat

Applications

PB9765 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

107.082kDa

Background of Mineralocorticoid R/NR3C2

NR3C2 (nuclear receptor subfamily 3, group C, member 2), also known as MR (mineralocorticoid receptor), is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. It belongs to the nuclear receptor family where the ligand diffuses into cells, interacts with the receptor and results in a signal transduction affecting specific gene expression in the nucleus. This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 2. IF analysis of NR3C2 using anti-NR3C2 antibody (PB9765) and anti-Tubulin Alpha antibody (M03989-3).
NR3C2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NR3C2 Antibody (PB9765) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 1. Western blot analysis of NR3C2 using anti-NR3C2 antibody (PB9765).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human LNCAP whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C2 antigen affinity purified polyclonal antibody (Catalog # PB9765) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C2 at approximately 110 kDa. The expected band size for NR3C2 is at 107 kDa.

Gene/Protein Information For NR3C2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ41052; MGC133092; Mineralocorticoid R; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MineralocorticoidR; MLR; MLRmineralocorticoid receptor; MRMCRaldosterone receptor; NR3C2; NR3C2VIT; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2 NR3C2 MCR, MLR, MRVIT, NR3C2 nuclear receptor subfamily 3 group C member 2 mineralocorticoid receptor|aldosterone receptor|mineralocorticoid receptor 1|mineralocorticoid receptor 2|mineralocorticoid receptor delta|nuclear receptor subfamily 3, group C, member 2 variant 3

For more info on NR3C2, check out the NR3C2 Infographic

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In this infographic, you will see the following information for NR3C2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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