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Product Info Summary
| SKU: | A09321-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NRAP Antibody Picoband®
SKU/Catalog Number
A09321-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NRAP Antibody Picoband® catalog # A09321-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NRAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09321-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NRAP recombinant protein (Position: M1-K1513). Human NRAP shares 86.8% amino acid (aa) sequence identity with mouse NRAP.
Reactive Species
A09321-1 is reactive to NRAP in Human, Mouse, Rat
Observed Molecular Weight
250 kDa
Calculated molecular weight
197.1 kDa
Background of NRAP
NRAP may be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09321-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat H9C2 whole cell, mouse C2C12 whole cell
Validation Images & Assay Conditions
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Western blot analysis of NRAP using anti-NRAP antibody (A09321-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat H9C2 whole cell lysates,
Lane 2: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAP antigen affinity purified polyclonal antibody (Catalog # A09321-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRAP at approximately 250 kDa. The expected band size for NRAP is at 197 kDa.
Specific Publications For Anti-NRAP Antibody Picoband® (A09321-1)
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