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Product Info Summary
| SKU: | A00505-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Nrl Antibody
SKU/Catalog Number
A00505-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Nrl Antibody catalog # A00505-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nrl Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00505-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NRL. AA range:19-68
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00505-1 is reactive to NRL in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
25.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00505-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Nrl Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of NRL Antibody. The lane on the right is blocked with the NRL peptide.
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Western blot analysis of the lysates from HUVECcells using NRL antibody.
Specific Publications For Anti-Nrl Antibody (A00505-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Nrl Antibody
Question
We are currently using anti-Nrl antibody A00505-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-Nrl antibody (A00505-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
Question
I was wanting to use your anti-Nrl antibody for WB for human retina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human retina identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
It shows on the product datasheet, A00505-1 anti-Nrl antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
Will A00505-1 anti-Nrl antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
It shows on the product datasheet, A00505-1 anti-Nrl antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-25
Question
Is there a BSA free version of anti-Nrl antibody A00505-1 available?
J. Lewis
Verified customer
Asked: 2019-03-04
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Nrl antibody A00505-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-04
Question
Would anti-Nrl antibody A00505-1 work on primate WB with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
Our lab technicians have not validated anti-Nrl antibody A00505-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Nrl antibody A00505-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pigmented layer of retina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-14
Question
I am interested in to test anti-Nrl antibody A00505-1 on human retina for research purposes, then I may be interested in using anti-Nrl antibody A00505-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
The products we sell, including anti-Nrl antibody A00505-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for retina using anti-Nrl antibody A00505-1. Let me know if you need anything else.
G. Johnson
Verified customer
Asked: 2014-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-08-06
