Anti-Nrl Antibody

NRL antibody

Boster Bio Anti-Nrl Antibody catalog # A00505-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A00505-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-Nrl Antibody

View all NRL Antibodies

SKU/Catalog Number

A00505-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Nrl Antibody catalog # A00505-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nrl Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00505-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

8F6A9; 8H5C5

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human Nrl. at AA rangle: 10-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00505-1 is reactive to NRL in Human, Mouse

Applications

A00505-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

25.94kDa

Background of NRL

Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion

Zhang, J. et al. (2000) J. Biol. Chem. 275, 35442-35447.
Turner, M. et al. (2000) Immunol. Today 21, 148-154.
Decker, M. et al. (1998) J. Biol. Chem. 273, 8867-8874.
Law, C.L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For NRL (Source: Uniprot.org, NCBI)

Gene Name

NRL

Full Name

Neural retina-specific leucine zipper protein

Weight

25.94kDa

Superfamily

bZIP family

Alternative Names

D14S46Eneural retina-specific leucine zipper protein; neural retina leucine zipper; NRL; NRL-MAF; RP27; RP27neural retinal-specific leucine zipper NRL D14S46E-MAF, RP27, NRL neural retina leucine zipper neural retina-specific leucine zipper protein|neural retinal-specific leucine zipper

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NRL, check out the NRL Infographic

NRL infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Nrl Antibody

Question

We are currently using anti-Nrl antibody A00505-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

The anti-Nrl antibody (A00505-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-24

Question

I was wanting to use your anti-Nrl antibody for WB for human retina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human retina identification?

Verified Customer

Verified customer

Asked: 2019-10-25

Answer

It shows on the product datasheet, A00505-1 anti-Nrl antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human retina in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-25

Question

Will A00505-1 anti-Nrl antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

It shows on the product datasheet, A00505-1 anti-Nrl antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-25

Question

Is there a BSA free version of anti-Nrl antibody A00505-1 available?

J. Lewis

Verified customer

Asked: 2019-03-04

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Nrl antibody A00505-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-03-04

Question

Would anti-Nrl antibody A00505-1 work on primate WB with pigmented layer of retina?

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

Our lab technicians have not validated anti-Nrl antibody A00505-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Nrl antibody A00505-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate pigmented layer of retina in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-14

Question

I am interested in to test anti-Nrl antibody A00505-1 on human retina for research purposes, then I may be interested in using anti-Nrl antibody A00505-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

The products we sell, including anti-Nrl antibody A00505-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-08

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for retina using anti-Nrl antibody A00505-1. Let me know if you need anything else.

G. Johnson

Verified customer

Asked: 2014-08-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-08-06

Order DetailsPrice
A00505-1

100uL

$399

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