Anti-NSD1/Kmt3B Monoclonal Antibody

KMT3B/NSD1 antibody

Boster Bio Anti-NSD1/Kmt3B Monoclonal Antibody catalog # A02327. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A02327
Size: 100μl
Reactive Species: Human
Host: Mouse
Application: WB

Product Name

Anti-NSD1/Kmt3B Monoclonal Antibody

View all KMT3B/NSD1 Antibodies

SKU/Catalog Number

A02327

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NSD1/Kmt3B Monoclonal Antibody catalog # A02327. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NSD1/Kmt3B Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02327)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

1G11

Isotype

IgG

Immunogen

Purified recombinant human NSD1 protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02327 is reactive to Nsd1 in Human

Applications

A02327 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

284.084kDa

Background of KMT3B/NSD1

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse

Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105(10):3992-7.
Cnops L, et al. Cereb Cortex. 2008 May; 18(5):1221-31.
Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18(12):4957-68.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Nsd1 (Source: Uniprot.org, NCBI)

Gene Name

Nsd1

Full Name

Histone-lysine N-methyltransferase, H3 lysine-36 specific

Weight

284.084kDa

Superfamily

class V-like SAM-binding methyltransferase superfamily

Alternative Names

Androgen receptor coactivator 267 kDa protein; Androgen receptor-associated protein of 267 kDa; ARA267STO; DKFZp666C163; EC 2.1.1.43; FLJ10684; FLJ22263; FLJ44628; H3 lysine-36 and H4 lysine-20 specific; KMT3BSotos syndrome; Lysine N-methyltransferase 3B; NR-binding SET domain-containing protein; nuclear receptor binding SET domain protein 1 Nsd1|AI528500, KMT3B|nuclear receptor-binding SET-domain protein 1|histone-lysine N-methyltransferase, H3 lysine-36 specific|H3-K36-HMTase|H4-K20-HMTase|NR-binding SET domain-containing protein|histone-lysine N-methyltransferase, H3 lysine-36 and H4 lysine-20 specific|nuclear receptor-binding SET domain-containing protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Nsd1, check out the Nsd1 Infographic

Nsd1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Nsd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-NSD1/Kmt3B Monoclonal Antibody

Question

Will anti-NSD1/Kmt3B Monoclonal antibody A02327 work on feline WB with corpus callosum?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Our lab technicians have not tested anti-NSD1/Kmt3B Monoclonal antibody A02327 on feline. You can run a BLAST between feline and the immunogen sequence of anti-NSD1/Kmt3B Monoclonal antibody A02327 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline corpus callosum in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-29

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate cancer using anti-NSD1/Kmt3B Monoclonal antibody A02327. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-13

Question

We are currently using anti-NSD1/Kmt3B Monoclonal antibody A02327 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

K. Carter

Verified customer

Asked: 2015-07-10

Answer

The anti-NSD1/Kmt3B Monoclonal antibody (A02327) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-10

Order DetailsPrice
A02327

100uL

$399

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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