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Product Info Summary
| SKU: | A15877-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-NTMT2 Antibody Picoband®
SKU/Catalog Number
A15877-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NTMT2 Antibody Picoband® catalog # A15877-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NTMT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15877-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human NTMT2 recombinant protein (Position: M1-S283). Human NTMT2 shares 93.6% and 93.3% amino acid (aa) sequence identity with mouse and rat NTMT2, respectively.
Reactive Species
A15877-1 is reactive to NTMT2 in Human, Rat
Observed Molecular Weight
32 kDa
Calculated molecular weight
32.4 kDa
Background of NTMT2
Enables N-terminal protein N-methyltransferase activity. Involved in N-terminal protein amino acid methylation. Located in nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15877-1 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Rat |
| ELISA | 0.1-0.5 μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of NTMT2 using anti-NTMT2 antibody (A15877-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTMT2 antigen affinity purified polyclonal antibody (Catalog # A15877-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTMT2 at approximately 32 kDa. The expected band size for NTMT2 is at 32 kDa.
Specific Publications For Anti-NTMT2 Antibody Picoband® (A15877-1)
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