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NTN3 antibody (A17692) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of NTN3 using anti-NTN3 antibody (A17692).
NTN3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NTN3 Antibody (A17692) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
NTN3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NTN3 Antibody (A17692) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-NTN3 Antibody Picoband®
NTN3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
NTN3 antibody was positively detected by WB in:
human RT4 whole cell, human U251 whole cell, human SH-SY5Y whole cell, human Hela whole cell, rat liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NTN3 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NTN3 antibody was positively detected by ICC/IF in:
HELA cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NTN3 Antibody Picoband®
View all NTN3 Antibodies
View all NTN3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NTN3 recombinant protein (Position: R51-R473).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
61.5 kDa
Background of NTN3
Netrins are a family of highly conserved proteins responsible for axon guidance and cell movement throughout neural development. Netrins can be divided into secreted netrins (netrin 1,3,4 and 5) and membrane-tethered glycophosphatidylinositol (GPI)-linked netrins (netrin G1 and G2).Secreted netrins carry their function via interaction with several receptors that include the deleted in colorectal (DCC) family, and the uncoordinated-5 (UNC5-A through UNC5-D) family.Netrin-3 was discovered in 1997 using sequence homology searching of netrin-2. Similarly to other netrins, Netrin-3 plays an important role in the development of the nervous system. Netrin-3 structure consist of a laminin-like domain located on the N-terminal, three epidermal growth factors like repeats (EGF), and a C-terminal netrin-like domain (NTR). Mutations in NTN3, the gene encoding netrin-3 was found to be associated with the development of several carcinomas.
Cite This Product
Anti-NTN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17692)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
91%
High Homology
Cow
91%
High Homology
Sheep
90%
High Homology
Mouse
88%
Moderate Homology
Rat
88%
Moderate Homology
Rabbit
66%
Low homology
Zebrafish
63%
Low homology
Drosophila
55%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NTN3 antibody
Immunohistochemistry (IHC) protocol for NTN3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NTN3 antibody
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Product Promise
We promise Anti-NTN3 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A17692
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026