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Product Info Summary
| SKU: | A02137-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, ICC, WB |
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Product info
Product Name
Anti-Nuclear RNA export factor 1 NXF1 Antibody
SKU/Catalog Number
A02137-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Nuclear RNA export factor 1 NXF1 Antibody catalog # A02137-1. Tested in WB,ICC,IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nuclear RNA export factor 1 NXF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02137-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CtBP1 around the phosphorylation site of S422.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02137-1 is reactive to NXF1 in Human
Applications
A02137-1 is guaranteed for IP, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
70.182kDa
Background of NXF1
Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2) By similarity. Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth.
Hateboer G., EMBO J. 14:3159-3169(1995).
Deloukas P., Nature 429:375-381(2004).
Matsuoka S., Science 316:1160-1166(2007).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:100
IP: 1:10-1:50
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of NXF1 using Anti-Nuclear RNA export factor 1 NXF1 Antibody (A02137-1).
ICC staining NXF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Nuclear RNA export factor 1 NXF1 Antibody A02137-1
Western blot analysis of NXF1 on 293 (1) and SiHa (2) cell lysate using anti-NXF1 antibody at 1/2Protein Target Info & Infographic
Gene/Protein Information For NXF1 (Source: Uniprot.org, NCBI)
Gene Name
NXF1
Full Name
Nuclear RNA export factor 1
Weight
70.182kDa
Superfamily
NXF family
Alternative Names
DKFZp667O0311; Mex67; mRNA export factor TAP; nuclear RNA export factor 1; TAPMEX67; tip associating protein; Tip-associated protein; Tip-associating protein NXF1 MEX67, TAP nuclear RNA export factor 1 nuclear RNA export factor 1|mRNA export factor TAP|tip associating protein|tip-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NXF1, check out the NXF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NXF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Nuclear RNA export factor 1 NXF1 Antibody (A02137-1)
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No publications found for A02137-1
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