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Product Info Summary
| SKU: | A05600 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Nucleolysin TIAR TIAL1 Antibody
SKU/Catalog Number
A05600
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Nucleolysin TIAR TIAL1 Antibody catalog # A05600. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Nucleolysin TIAR TIAL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05600)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human TIAL1(NP_003243.1).
Reactive Species
A05600 is reactive to TIAL1 in Human, Mouse, Rat
Observed Molecular Weight
111 kDa
Calculated molecular weight
41.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05600 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:100-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using TIAL1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat lung using TIAL1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded human appendix using TIAL1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded mouse spleen using TIAL1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Specific Publications For Anti-Nucleolysin TIAR TIAL1 Antibody (A05600)
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