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Product Info Summary
| SKU: | A08301-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-NUDT6 Antibody Picoband®
SKU/Catalog Number
A08301-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-NUDT6 Antibody Picoband® catalog # A08301-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-NUDT6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08301-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUDT6 recombinant protein (Position: D45-K303).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08301-1 is reactive to NUDT6 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.7 kDa
Background of NUDT6
Nucleoside diphosphate-linked moiety X motif 6 is a protein that in humans is encoded by the NUDT6 gene. This gene overlaps and lies on the opposite strand from FGF2 gene, and is thought to be the FGF2 antisense gene. The two genes are independently transcribed, and their expression shows an inverse relationship, suggesting that this antisense transcript may regulate FGF2 expression. This gene has also been shown to have hormone-regulatory and antiproliferative actions in the pituitary that are independent of FGF2 expression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08301-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human HepG2 whole cell, human K562 whole cell, human THP-1 whole cell, human PC-3 whole cell, human A549 whole cell, human Hela whole cell, human SH-SY5Y whole cell, rat kidney tissue, rat H9C2 whole cell, mouse kidney tissue, mouse HEPA1/6 whole cell
IHC: human liver cancer tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of NUDT6 using anti-NUDT6 antibody (A08301-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: human SH-SY5Y whole cell lysates,
Lane 9: rat kidney tissue lysates,
Lane 10: rat H9C2 whole cell lysates,
Lane 11: mouse kidney tissue lysates,
Lane 12: mouse HEPA1/6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT6 antigen affinity purified polyclonal antibody (Catalog # A08301-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT6 at approximately 72 kDa. The expected band size for NUDT6 is at 72 kDa.
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IHC analysis of NUDT6 using anti-NUDT6 antibody (A08301-1).
NUDT6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUDT6 Antibody (A08301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of NUDT6 using anti-NUDT6 antibody (A08301-1).
NUDT6 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUDT6 Antibody (A08301-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-NUDT6 Antibody Picoband® (A08301-1)
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