Anti-NUFIP1 Antibody Picoband™

Product Name

Anti-NUFIP1 Antibody Picoband™

View all NUFIP1 Antibodies

SKU/Catalog Number

A10137-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-NUFIP1 Antibody Picoband™ catalog # A10137-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-NUFIP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10137-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NUFIP1 recombinant protein (Position: Q66-D481).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10137-1 is reactive to NUFIP1 in Human

Applications

A10137-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

56.3kDa

Background of NUFIP1

Nuclear fragile X mental retardation-interacting protein 1 is a protein that in humans is encoded by the NUFIP1 gene. This gene encodes a nuclear RNA binding protein that contains a C2H2 zinc finger motif and a nuclear localization signal. This protein is associated with the nuclear matrix in perichromatin fibrils and, in neurons, localizes to the cytoplasm in association with endoplasmic reticulum ribosomes. This protein interacts with the fragile X mental retardation protein (FMRP), the tumor suppressor protein BRCA1, upregulates RNA polymerase II transcription, and is involved in box C/D snoRNP biogenesis. A pseudogene of this gene resides on chromosome 6q12.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of NUFIP1 using anti-NUFIP1 antibody (A10137-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: human 293T whole cell lysates,
Lane 6: human HepG2 whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUFIP1 antigen affinity purified polyclonal antibody (Catalog # A10137-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUFIP1 at approximately 75 kDa. The expected band size for NUFIP1 is at 75 kDa.

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Figure 2. IF analysis of NUFIP1 using anti-NUFIP1 antibody (A10137-1) and anti-Beta Tubulin antibody (M01857-3).
NUFIP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUFIP1 Antibody (A10137-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For NUFIP1 (Source: Uniprot.org, NCBI)

Alternative Names

bA540M5.1; nuclear fragile X mental retardation protein interacting protein 1; nuclear fragile X mental retardation protein-interacting protein 1; nuclear fragile X mental retardation-interacting protein 1; NUFIP; NUFIPNuclear FMRP-interacting protein 1 NUFIP1 NUFIP, Rsa1, bA540M5.1 nuclear FMR1 interacting protein 1 nuclear fragile X mental retardation-interacting protein 1|NUFIP1, FMR1 interacting protein 1|nuclear FMRP-interacting protein 1|nuclear fragile X mental retardation protein interacting protein 1

For more info on NUFIP1, check out the NUFIP1 Infographic

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In this infographic, you will see the following information for NUFIP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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