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Product Info Summary
| SKU: | M02183 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-NUP153 Monoclonal Antibody
SKU/Catalog Number
M02183
BM5703 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NUP153 Monoclonal Antibody catalog # M02183. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NUP153 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02183)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFDA-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Nup153 Possible DNA-binding subunit of the nuclear pore complex (NPC) . The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Reactive Species
M02183 is reactive to NUP153 in Human
Observed Molecular Weight
154 kDa
Calculated molecular weight
153.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02183 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of Nup153 expression in K562 cell lysate.
Specific Publications For Anti-NUP153 Monoclonal Antibody (M02183)
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Customer Q&As
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2 Customer Q&As for Anti-NUP153 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02183?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Nup153 expression in K562 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We are currently using anti-NUP153 Monoclonal antibody M02183 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The anti-NUP153 Monoclonal antibody (M02183) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-11
