Anti-NUP160 Antibody

Product Name

Anti-NUP160 Antibody

View all NUP160 Antibodies

SKU/Catalog Number

A05629-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NUP160 Antibody (Catalog # A05629-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

NUP160 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NUP160 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05629-1)

Host

Rabbit

Contents

NUP160 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NUP160 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human NUP160. The immunogen is located within the last 50 amino acids of NUP160.

Reactive Species

A05629-1 is reactive to NUP160 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

162.1 kDa

Background of NUP160

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05629-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NUP160 antibody can be used for detection of NUP160 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NUP160

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