This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
NUP214 antibody (A02408) performs well in ELISA, Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Flow Cytometry analysis of HL-60 cells using anti-NUP214 antibody (A02408).
Overlay histogram showing HL-60 cells stained with A02408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP214 Antibody (A02408, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HL-60 cells stained with A02408 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP214 Antibody (A02408, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-NUP214 Antibody Picoband®
NUP214 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, Flow Cytometry, IF, ICC, WB |
Tested Applications
NUP214 antibody was positively detected by WB in:
human Hela whole cell, human HEK293 whole cell, human K562 whole cell, human THP-1 whole cell, mouse spleen tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NUP214 antibody was positively detected by ICC/IF in:
A431 cell, NIH/3T3 cell
NUP214 antibody was positively detected by FC in:
HL-60 cell, HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse
Immunocytochemistry/Immunofluorescence
2μg/ml
Human, Mouse
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human, Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
ELISA
Human
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Rabbit
Predicted
Predicted
Bovine
Predicted
Predicted
Sheep
Predicted
Predicted
Product Information
Product Name
Anti-NUP214 Antibody Picoband®
View all NUP214 Antibodies
View all NUP214 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NUP214 recombinant protein (Position: K36-E374).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
280 kDa
Calculated Molecular Weight
213.6 kDa
Background of NUP214
Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t (6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-NUP214 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02408)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
97%
High Homology
Rabbit
96%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Mouse
88%
Moderate Homology
Rat
87%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NUP214 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for NUP214 antibody
Flow Cytometry (FC) protocol for NUP214 antibody
Review
Have you used Anti-NUP214 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-NUP214 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A02408
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026