Anti-OCT1/Slc22a1 Antibody Picoband™

Product Name

Anti-OCT1/Slc22a1 Antibody Picoband™

View all SLC22A1 Antibodies

SKU/Catalog Number

A01945-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-OCT1/Slc22a1 Antibody Picoband™ catalog # A01945-1. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OCT1/Slc22a1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01945-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse OCT1/Slc22a1, which shares 66.7% and 94.4% amino acid (aa) sequence identity with human and rat SLC22A1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01945-1 is reactive to SLC22A1 in Mouse

Applications

A01945-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

61.154kDa

Background of SLC22A1

Solute carrier family 22(organic cation transporter), member 1, also called SLC22A1 or OCT1 is a protein that in humans is encoded by the SLC22A1 gene. This gene is mapped to 6q25.3. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene transports the polyamines spermine and spermidine. It also transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. This gene regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OCT1/Slc22a1 using anti-OCT1/Slc22a1 antibody (A01945-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCT1/Slc22a1 antigen affinity purified polyclonal antibody (Catalog # A01945-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCT1/Slc22a1 at approximately 75 kDa. The expected band size for OCT1/Slc22a1 is at 62 kDa.

Gene/Protein Information For SLC22A1 (Source: Uniprot.org, NCBI)

Alternative Names

hOCT1; OCT1; oct1_cds; OCT1solute carrier family 22 member 1; Organic cation transporter 1; SLC22A1; solute carrier family 22 (organic cation transporter), member 1 SLC22A1 HOCT1, OCT1, oct1_cds solute carrier family 22 member 1 solute carrier family 22 member 1|organic cation transporter 1|solute carrier family 22 (organic cation transporter), member 1

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In this infographic, you will see the following information for SLC22A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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