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Slc22a1 antibody (A01945-1) performs well in WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of OCT1/Slc22a1 using anti-OCT1/Slc22a1 antibody (A01945-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCT1/Slc22a1 antigen affinity purified polyclonal antibody (Catalog # A01945-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCT1/Slc22a1 at approximately 75 kDa. The expected band size for OCT1/Slc22a1 is at 62 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse HEPA1-6 whole cell lysates.
red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCT1/Slc22a1 antigen affinity purified polyclonal antibody (Catalog # A01945-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCT1/Slc22a1 at approximately 75 kDa. The expected band size for OCT1/Slc22a1 is at 62 kDa.
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Anti-OCT1/Slc22a1 Antibody Picoband®
Slc22a1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | WB |
Tested Applications
Slc22a1 antibody was positively detected by WB in:
mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-OCT1/Slc22a1 Antibody Picoband®
View all SLC22A1 Antibodies
View all SLC22A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse OCT1/Slc22a1, which shares 66.7% and 94.4% amino acid (aa) sequence identity with human and rat SLC22A1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
61.5 kDa
Background of Slc22a1
Solute carrier family 22(organic cation transporter), member 1, also called SLC22A1 or OCT1 is a protein that in humans is encoded by the SLC22A1 gene. This gene is mapped to 6q25.3. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene transports the polyamines spermine and spermidine. It also transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. This gene regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin-dependent kinase II and LCK tyrosine kinase.
Cite This Product
Anti-OCT1/Slc22a1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01945-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
90%
High Homology
Rabbit
70%
Moderate Homology
Monkey
70%
Moderate Homology
Human
65%
Low homology
Cow
65%
Low homology
Sheep
65%
Low homology
Dog
60%
Low homology
Zebrafish
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Slc22a1 antibody
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A01945-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 25, 2026