Anti-OCT4/POU5F1 Antibody (Monoclonal, 31P08)

Product Name

Anti-OCT4/POU5F1 Antibody (Monoclonal, 31P08)

View all POU5F1 Antibodies

SKU/Catalog Number

M00174-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-OCT4/POU5F1 Antibody (Monoclonal, 31P08) catalog # M00174-3. Tested in WB, IHC, IF, ICC/IF, IP, Flow Cytometry, ChIP applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-OCT4/POU5F1 Antibody (Monoclonal, 31P08) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00174-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

31P08

Immunogen

Synthetic peptide within human Oct4 aa 22-58.

Reactive Species

M00174-3 is reactive to POU5F1 in Human, Mouse

Calculated molecular weight

38.6 kDa

Background of POU5F1

Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. It is mapped to 6p21.33. This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00174-3 is guaranteed for ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200
Immunofluorescence, 1:50-200
Immunocytochemistry/Immunofluorescence, 1:50-200
ImmunoPrecipitation, 1:50
Flow Cytometry (Fixed), 1:50-200

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information POU5F1

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