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Product Info Summary
| SKU: | M00174 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00174
BM4618 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody catalog # M00174. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00174)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GDI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Oct4
Reactive Species
M00174 is reactive to POU5F1 in Human, Mouse
Observed Molecular Weight
45 kDa
Calculated molecular weight
38.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00174 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human seminoma tissue
ICC/IF: NCCIT cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2k dilution for 1 hour at room temperature.
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Immunofluorescent analysis of NCCIT cells, using Oct4 Antibody.
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Western blot analysis of Oct4 expression in NCCIT cell lysate.
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Immunohistochemical analysis of paraffin-embedded human seminoma, using Oct4 Antibody.
Specific Publications For Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody (M00174)
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Customer Q&As
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3 Customer Q&As for Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for blood using anti-Oct4 Rabbit Monoclonal antibody M00174. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-09
Question
Would anti-Oct4 Rabbit Monoclonal antibody M00174 work on goat IP with adult mammalian kidney?
Verified Customer
Verified customer
Asked: 2018-10-12
Answer
Our lab technicians have not tested anti-Oct4 Rabbit Monoclonal antibody M00174 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Oct4 Rabbit Monoclonal antibody M00174 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat adult mammalian kidney in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-12
Question
Would anti-Oct4 Rabbit Monoclonal antibody M00174 work for ChIP with blood?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
According to the expression profile of blood, POU5F1 is highly expressed in blood. So, it is likely that anti-Oct4 Rabbit Monoclonal antibody M00174 will work for ChIP with blood.
Boster Scientific Support
Answered: 2017-10-16
