Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody

POU5F1 antibody

Boster Bio Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody catalog # M00174. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse. Cited in 2 publication(s).

Product Info Summary

SKU: M00174
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

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Product Name

Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody

View all POU5F1 Antibodies

SKU/Catalog Number

M00174

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody catalog # M00174. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00174)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GDI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Oct4

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00174 is reactive to POU5F1 in Human, Mouse

Applications

M00174 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

29 kDa

Calculated molecular weight

38.571kDa

Background of POU5F1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For POU5F1 (Source: Uniprot.org, NCBI)

Gene Name

POU5F1

Full Name

POU domain, class 5, transcription factor 1

Weight

38.571kDa

Superfamily

POU transcription factor family

Alternative Names

class 5, transcription factor 1; MGC22487; Oct-3; OCT3Oct4; Oct-4; Octamer-binding protein 3; otc-4; OTF3POU domain class 5, transcription factor 1; OTF4; POU class 5 homeobox 1; POU-type homeodomain-containing DNA-binding protein POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 POU class 5 homeobox 1 POU domain, class 5, transcription factor 1|POU domain transcription factor OCT4|POU-type homeodomain-containing DNA-binding protein|octamer-binding protein 3|octamer-binding protein 4|octamer-binding transcription factor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on POU5F1, check out the POU5F1 Infographic

POU5F1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for POU5F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00174 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells

Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

Have you used Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody?

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3 Customer Q&As for Anti-Oct4 POU5F1 Rabbit Monoclonal Antibody

Question

Here is the WB image, lot number and protocol we used for blood using anti-Oct4 Rabbit Monoclonal antibody M00174. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-09

Question

Would anti-Oct4 Rabbit Monoclonal antibody M00174 work on goat IP with adult mammalian kidney?

Verified Customer

Verified customer

Asked: 2018-10-12

Answer

Our lab technicians have not tested anti-Oct4 Rabbit Monoclonal antibody M00174 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Oct4 Rabbit Monoclonal antibody M00174 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat adult mammalian kidney in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-12

Question

Would anti-Oct4 Rabbit Monoclonal antibody M00174 work for ChIP with blood?

Verified Customer

Verified customer

Asked: 2017-10-16

Answer

According to the expression profile of blood, POU5F1 is highly expressed in blood. So, it is likely that anti-Oct4 Rabbit Monoclonal antibody M00174 will work for ChIP with blood.

Boster Scientific Support

Answered: 2017-10-16

Order DetailsPrice
M00174

100μL

$370
M00174-30ul

30μL sample

$99
M00174-carrier-free

Carrier Free

$370

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