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Product Info Summary
| SKU: | A05301-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-OGDH Antibody Picoband™
SKU/Catalog Number
A05301-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OGDH Antibody Picoband™ catalog # A05301-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OGDH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05301-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OGDH recombinant protein (Position: M1-S1023).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05301-1 is reactive to OGDH in Human, Mouse, Rat
Applications
A05301-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
110 kDa
Calculated molecular weight
115.935kDa
Background of OGDH
Alpha-ketoglutarate dehydrogenase also known as 2-oxoglutarate dehydrogenase E1 component, mitochondrial is an enzyme that in humans is encoded by the OGDH gene. This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OGDH using anti-OGDH antibody (A05301-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: rat heart tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGDH antigen affinity purified polyclonal antibody (Catalog # A05301-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGDH at approximately 110 kDa. The expected band size for OGDH is at 116 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-OGDH antibody (A05301-1).
Overlay histogram showing Daudi cells stained with A05301-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGDH Antibody (A05301-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For OGDH (Source: Uniprot.org, NCBI)
Gene Name
OGDH
Full Name
2-oxoglutarate dehydrogenase, mitochondrial
Weight
115.935kDa
Superfamily
alpha-ketoglutarate dehydrogenase family
Alternative Names
2-oxoglutarate dehydrogenase complex component E1; 2-oxoglutarate dehydrogenase, mitochondrial; AKGDH; alpha KGD; alpha-KGD; E1k; OGDC; oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide); oxoglutarate decarboxylase; oxoglutarate dehydrogenase (succinyl-transferring) OGDH AKGDH, E1k, KGD1, OGDC2, OGDH oxoglutarate dehydrogenase 2-oxoglutarate dehydrogenase, mitochondrial|2-oxoglutarate dehydrogenase complex component E1|OGDC-E1|oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)|oxoglutarate decarboxylase|oxoglutarate dehydrogenase (succinyl-transferring)|testicular tissue protein Li 131
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OGDH, check out the OGDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OGDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OGDH Antibody Picoband™ (A05301-1)
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