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Product Info Summary
| SKU: | A10713-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-OGDHL Antibody Picoband™
SKU/Catalog Number
A10713-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-OGDHL Antibody Picoband™ catalog # A10713-1. Tested in ELISA, IF, ICC, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-OGDHL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10713-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OGDHL recombinant protein (Position: M1-F1010).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10713-1 is reactive to OGDHL in Human, Mouse, Rat
Applications
A10713-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
100 kDa
Calculated molecular weight
114.481kDa
Background of OGDHL
The protein encoded by this gene is similar to oxoglutarate dehydrogenase (OGDH) of the OGDH complex, which degrades glucose and glutamate. This gene encodes several isoforms, including some that appear to localize to mitochondria. The encoded protein down-regulates the AKT signaling cascade and can suppress the growth of cervical cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of OGDHL using anti-OGDHL antibody (A10713-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGDHL antigen affinity purified polyclonal antibody (Catalog # A10713-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGDHL at approximately 100 kDa. The expected band size for OGDHL is at 114 kDa.
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Figure 2. IF analysis of OGDHL using anti-OGDHL antibody (A10713-1).
OGDHL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OGDHL Antibody (A10713-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of U937 cells using anti-OGDHL antibody (A10713-1).
Overlay histogram showing U937 cells stained with A10713-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGDHL Antibody (A10713-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For OGDHL (Source: Uniprot.org, NCBI)
Gene Name
OGDHL
Full Name
2-oxoglutarate dehydrogenase-like, mitochondrial
Weight
114.481kDa
Superfamily
alpha-ketoglutarate dehydrogenase family
Alternative Names
mitochondrial; OGDC-E1-like; oxoglutarate dehydrogenase-like OGDHL oxoglutarate dehydrogenase L 2-oxoglutarate dehydrogenase-like, mitochondrial|2-oxoglutarate dehydrogenase complex component E1-like|OGDC-E1-like|alpha-ketoglutarate dehydrogenase-like|oxoglutarate dehydrogenase like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OGDHL, check out the OGDHL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OGDHL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OGDHL Antibody Picoband™ (A10713-1)
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