Anti-OLAH Antibody Picoband™

Product Name

Anti-OLAH Antibody Picoband™

View all OLAH Antibodies

SKU/Catalog Number

A05069-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Anti-OLAH Antibody Picoband™ Antibody Picoband™ catalog # A05069-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-OLAH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05069-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human OLAH recombinant protein (Position: E13-F265).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05069-3 is reactive to OLAH in Human

Applications

A05069-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

36 kDa

Calculated molecular weight

29.931kDa

Background of OLAH

Enables dodecanoyl-[acyl-carrier-protein] hydrolase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; and palmitoyl-[acyl-carrier-protein] hydrolase activity. Involved in medium-chain fatty acid biosynthetic process. Predicted to be located in cytosol.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of OLAH using anti-OLAH antibody (A05069-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLAH antigen affinity purified polyclonal antibody (Catalog # A05069-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLAH at approximately 36 kDa. The expected band size for OLAH is at 35 kDa.

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Figure 2. IF analysis of OGDHL using anti-OGDHL antibody (A05069-3).
OGDHL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OGDHL Antibody (A05069-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of HEL cells using anti-OLAH antibody (A05069-3).
Overlay histogram showing HEL cells stained with A05069-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OLAH Antibody (A05069-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OLAH (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.2.14; FLJ11106; medium chain; MGC51852; oleoyl-ACP hydrolaseAURA1; SAST; THEDC1; thioesterase domain containing 1; Thioesterase II OLAH AURA1, SAST, TE2, THEDC1 oleoyl-ACP hydrolase S-acyl fatty acid synthase thioesterase, medium chain|augmented in rheumatoid arthritis 1|oleoyl-acyl-carrier-protein hydrolase|thioesterase 2|thioesterase II|thioesterase domain containing 1|thioesterase domain-containing protein 1

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In this infographic, you will see the following information for OLAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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