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Product Info Summary
| SKU: | A15315 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Olfactory receptor 2A25 Antibody
SKU/Catalog Number
A15315
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 2A25 Antibody catalog # A15315. Tested in WB, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 2A25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15315)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human OR2A25. AA range:241-290
Reactive Species
A15315 is reactive to OR2A25 in Human
Calculated molecular weight
34.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15315 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Olfactory receptor 2A25 Polyclonal Antibody
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Western blot analysis of lysates from HT-29 cells, using OR2A25 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using OR2A25 antibody.
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Immunofluorescence analysis of A549 cells, using OR2A25 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Olfactory receptor 2A25 Antibody (A15315)
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4 Customer Q&As for Anti-Olfactory receptor 2A25 Antibody
Question
I see that the anti-Olfactory receptor 2A25 antibody A15315 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-16
Question
My question regarding product A15315, anti-Olfactory receptor 2A25 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15315 anti-Olfactory receptor 2A25 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-05
Question
Would A15315 anti-Olfactory receptor 2A25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Huang
Verified customer
Asked: 2019-02-25
Answer
You can see on the product datasheet, A15315 anti-Olfactory receptor 2A25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-25
Question
Is this A15315 anti-Olfactory receptor 2A25 antibody reactive to the isotypes of OR2A25?
W. Jackson
Verified customer
Asked: 2017-05-15
Answer
The immunogen of A15315 anti-Olfactory receptor 2A25 antibody is Synthesized peptide derived from the C-terminal region of human Olfactory receptor 2A25. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-05-15
