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Product Info Summary
| SKU: | A17774 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Olfactory receptor 4X1 Antibody
SKU/Catalog Number
A17774
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 4X1 Antibody catalog # A17774. Tested in WB, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 4X1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17774)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human OR4X1. AA range:256-305
Reactive Species
A17774 is reactive to OR4X1 in Human
Calculated molecular weight
34.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17774 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Olfactory receptor 4X1 Polyclonal Antibody
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Western Blot analysis of COLO205 cells using Olfactory receptor 4X1 Polyclonal Antibody
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Western blot analysis of lysates from HeLa, Jurkat, HUVEC, MCF-7, and COLO cells, using OR4X1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using OR4X1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Olfactory receptor 4X1 Antibody (A17774)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Olfactory receptor 4X1 Antibody
Question
Is this A17774 anti-Olfactory receptor 4X1 antibody reactive to the isotypes of OR4X1?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
The immunogen of A17774 anti-Olfactory receptor 4X1 antibody is Synthesized peptide derived from the C-terminal region of human Olfactory receptor 4X1. at AA rangle: 220-300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-Olfactory receptor 4X1 antibody A17774 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Olfactory receptor 4X1 antibody (A17774) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
Will A17774 anti-Olfactory receptor 4X1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
You can see on the product datasheet, A17774 anti-Olfactory receptor 4X1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-25
Question
Is a blocking peptide available for product anti-Olfactory receptor 4X1 antibody (A17774)?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
We do provide the blocking peptide for product anti-Olfactory receptor 4X1 antibody (A17774). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-08-16
Question
Is there a BSA free version of anti-Olfactory receptor 4X1 antibody A17774 available?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Olfactory receptor 4X1 antibody A17774 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-13
Question
I see that the anti-Olfactory receptor 4X1 antibody A17774 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-14
Question
I have a question about product A17774, anti-Olfactory receptor 4X1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-25
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17774 anti-Olfactory receptor 4X1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-25
