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Product Info Summary
| SKU: | A17771 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Olfactory receptor 5W2 Antibody
SKU/Catalog Number
A17771
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 5W2 Antibody catalog # A17771. Tested in WB, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 5W2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17771)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human OR5W2. AA range:158-207
Reactive Species
A17771 is reactive to OR5W2 in Human
Calculated molecular weight
35.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A17771 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of 293T-UV cells using Olfactory receptor 5W2 Polyclonal Antibody diluted at 1:500
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Western blot analysis of lysates from HeLa cells, using OR5W2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using OR5W2 antibody.
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Immunofluorescence analysis of A549 cells, using OR5W2 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Olfactory receptor 5W2 Antibody (A17771)
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5 Customer Q&As for Anti-Olfactory receptor 5W2 Antibody
Question
We are currently using anti-Olfactory receptor 5W2 antibody A17771 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The anti-Olfactory receptor 5W2 antibody (A17771) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-17
Question
Do you have a BSA free version of anti-Olfactory receptor 5W2 antibody A17771 available?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Olfactory receptor 5W2 antibody A17771 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-09
Question
Will A17771 anti-Olfactory receptor 5W2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-20
Answer
It shows on the product datasheet, A17771 anti-Olfactory receptor 5W2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-20
Question
My question regarding product A17771, anti-Olfactory receptor 5W2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A17771 anti-Olfactory receptor 5W2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-16
Question
I see that the anti-Olfactory receptor 5W2 antibody A17771 works with WB, what is the protocol used to produce the result images on the product page?
B. Thomas
Verified customer
Asked: 2014-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-02-04
