Product Info Summary
SKU: | A12941 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Olfactory receptor 8J1 Antibody
SKU/Catalog Number
A12941
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Olfactory receptor 8J1 Antibody catalog # A12941. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olfactory receptor 8J1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12941)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2H4
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human Olfactory receptor 8J1. at AA rangle: 200-280
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12941 is reactive to OR8J1 in Human
Applications
A12941 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
35.415kDa
Background of OR8J1
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of Jurkat cells using Olfactory receptor 8J1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For OR8J1 (Source: Uniprot.org, NCBI)
Gene Name
OR8J1
Full Name
Olfactory receptor 8J1
Weight
35.415kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Olfactory receptor 8J1 OR8J1 OR11-183 olfactory receptor family 8 subfamily J member 1 olfactory receptor 8J1|olfactory receptor OR11-183
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on OR8J1, check out the OR8J1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for OR8J1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Olfactory receptor 8J1 Antibody (A12941)
Hello CJ!
No publications found for A12941
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-Olfactory receptor 8J1 Antibody
Question
My question regarding product A12941, anti-Olfactory receptor 8J1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12941 anti-Olfactory receptor 8J1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-27
Question
Would anti-Olfactory receptor 8J1 antibody A12941 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
According to the expression profile of testis, OR8J1 is highly expressed in testis. So, it is likely that anti-Olfactory receptor 8J1 antibody A12941 will work for WB with testis.
Boster Scientific Support
Answered: 2019-07-09
Question
I was wanting to use your anti-Olfactory receptor 8J1 antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can see on the product datasheet, A12941 anti-Olfactory receptor 8J1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Do you have a BSA free version of anti-Olfactory receptor 8J1 antibody A12941 available?
W. Huang
Verified customer
Asked: 2016-02-18
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Olfactory receptor 8J1 antibody A12941 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-18
Question
We are currently using anti-Olfactory receptor 8J1 antibody A12941 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
T. Rodriguez
Verified customer
Asked: 2015-09-17
Answer
The anti-Olfactory receptor 8J1 antibody (A12941) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-17
Question
Is a blocking peptide available for product anti-Olfactory receptor 8J1 antibody (A12941)?
L. Evans
Verified customer
Asked: 2013-11-07
Answer
We do provide the blocking peptide for product anti-Olfactory receptor 8J1 antibody (A12941). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-11-07