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- Table of Contents
TSC2 antibody (M00229-1) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A549 cells using anti-TSC2 antibody (M00229-1).
Overlay histogram showing A549 cells stained with M00229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TSC2 Antibody (M00229-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A549 cells stained with M00229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-TSC2 Antibody (M00229-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3)
TSC2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | Flow Cytometry, IHC, WB |
Tested Applications
TSC2 antibody was positively detected by WB in:
human HEK293 tissue, human PC-3 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TSC2 antibody was positively detected by IHC in:
human liver cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TSC2 antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3)
View all TSC2 Antibodies
View all TSC2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6I3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807). Human Tuberin shares 94% and 90% amino acid (aa) sequence identity with mouse and rat Tuberin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
280 kDa
Calculated Molecular Weight
200.6 kDa
Background of TSC2
Tuberous sclerosis 2 protein, also known as TSC2 or Tuberin is a protein that is in humans. The chromosomal location of this gene is 16p13.3. Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. This gene involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. It stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro.
Cite This Product
Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00229-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Mouse
91%
High Homology
Rabbit
91%
High Homology
Rat
90%
High Homology
Zebrafish
67%
Low homology
Dog
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TSC2 antibody
Immunohistochemistry (IHC) protocol for TSC2 antibody
Flow Cytometry (FC) protocol for TSC2 antibody
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Product Promise
We promise Anti-Tuberin TSC2 Antibody Picoband® (monoclonal, 6I3) performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00229-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026