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Product Info Summary
| SKU: | M03505 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal Antibody
SKU/Catalog Number
M03505
BM4632 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal Antibody catalog # M03505. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03505)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GFC-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human OPRD1
Reactive Species
M03505 is reactive to OPRD1 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
40.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03505 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:20
Positive Control
WB: human U87MG whole cell, human U251 whole cell, human U2OS whole cell, rat brain tissue, rat spleen tissue, mouse brain tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of OPRD1 using anti-OPRD1 antibody (M03505).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87MG whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPRD1 antigen affinity purified monoclonal antibody (M03505) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OPRD1 at approximately 42 kDa. The expected band size for OPRD1 is at 40 kDa.
Specific Publications For Anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal Antibody (M03505)
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Customer Q&As
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7 Customer Q&As for Anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal Antibody
Question
We are currently using anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
J. Wu
Verified customer
Asked: 2020-05-06
Answer
The anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody (M03505) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-06
Question
I have attached the WB image, lot number and protocol we used for brain cortex corpus striatum using anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
Can you help my question with product M03505, anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03505 anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-24
Question
Does M03505 anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
It shows on the product datasheet, M03505 anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-10
Question
Does anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505 work for WB with brain cortex corpus striatum?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
According to the expression profile of brain cortex corpus striatum, OPRD1 is highly expressed in brain cortex corpus striatum. So, it is likely that anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505 will work for WB with brain cortex corpus striatum.
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody (M03505)?
D. Patel
Verified customer
Asked: 2019-03-25
Answer
We do provide the blocking peptide for product anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody (M03505). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-03-25
Question
I am interested in to test anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505 on rat brain cortex corpus striatum for research purposes, then I may be interested in using anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Jones
Verified customer
Asked: 2014-02-17
Answer
The products we sell, including anti-OPRD1/Delta Opioid Receptor Rabbit Monoclonal antibody M03505, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-17
