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Product Info Summary
| SKU: | M00952 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8)
SKU/Catalog Number
M00952
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8) catalog # M00952. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00952)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
3D8
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00952 is reactive to OPTN in Human, Mouse, Rat
Observed Molecular Weight
66 kDa
Calculated molecular weight
65.9 kDa
Background of OPTN
OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00952 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human U-87MG whole cell,, human U20S whole cell,, human placenta tissue,, human Hela whole cell,, human A549 whole cell,, human K562 whole cell,, rat C6 whole cell,, mouse Neuro-2a whole cell
FCM: U20S cell
Validation Images & Assay Conditions
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Western blot analysis of Optineurin/OPTN using anti-Optineurin/OPTN antibody (M00952).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates;
Lane 2: human U20S whole cell lysates;
Lane 3: human placenta tissue lysates;
Lane 4: human Hela whole cell lysates;
Lane 5: human A549 whole cell lysates;
Lane 6: human K562 whole cell lysates;
Lane 7: rat C6 whole cell lysates;
Lane 8: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Optineurin/OPTN antigen affinity purified monoclonal antibody (Catalog # M00952) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Optineurin/OPTN at approximately 66KD. The expected band size for Optineurin/OPTN is at 66KD.
Specific Publications For Anti-Optineurin/OPTN Antibody Picoband® (monoclonal, 3D8) (M00952)
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