Anti-Optineurin/OPTN Antibody Picoband™

Product Name

Anti-Optineurin/OPTN Antibody Picoband™

View all Optineurin Antibodies

SKU/Catalog Number

PB9343

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Optineurin/OPTN Antibody Picoband™ catalog # PB9343. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Optineurin/OPTN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9343)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9343 is reactive to OPTN in Human, Mouse, Rat

Applications

PB9343 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

65.922kDa

Background of Optineurin

OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Optineurin using anti-Optineurin antibody (PB9343).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: huamn HepG2 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissuel lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Optineurin antigen affinity purified polyclonal antibody (Catalog # PB9343) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Optineurin at approximately 75 kDa. The expected band size for Optineurin is at 66 kDa.

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Figure 2. IHC analysis of Optineurin using anti-Optineurin antibody (PB9343).
Optineurin was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of Optineurin using anti-Optineurin antibody (PB9343).
Optineurin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. IHC analysis of Optineurin using anti-Optineurin antibody (PB9343).
Optineurin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 5. Flow Cytometry analysis of A431 cells using anti-Optineurin antibody (PB9343).
Overlay histogram showing A431 cells stained with PB9343 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Optineurin Antibody (PB9343, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For OPTN (Source: Uniprot.org, NCBI)

Alternative Names

FIP2FIP-2; GLC1Eglaucoma 1, open angle, E (adult-onset); HIP7ALS12; Huntingtin interacting protein L; Huntingtin yeast partner L; Huntingtin-interacting protein 7; Huntingtin-interacting protein L; HYPLHIP-7; NEMO-related protein; NRPE3-14.7K-interacting protein; Optic neuropathy-inducing protein; optineurin; TFIIIA-INTP; Transcription factor IIIA-interacting protein; transcrption factor IIIA-interacting protein; tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP optineurin optineurin|E3-14.7K-interacting protein|FIP-2|HIP-7|Huntingtin interacting protein L|huntingtin yeast partner L|huntingtin-interacting protein 7|huntingtin-interacting protein L|nemo-related protein|optic neuropathy-inducing protein|transcription factor IIIA-interacting protein|transcrption factor IIIA-interacting protein|tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains

For more info on OPTN, check out the OPTN Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for OPTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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