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Product Info Summary
| SKU: | A30873 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-OR4F4/4F5/4F17 Antibody
SKU/Catalog Number
A30873
Size
100ul
Form
Liquid
Description
Boster Bio Anti-OR4F4/4F5/4F17 Antibody (Catalog# A30873). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OR4F4/4F5/4F17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30873)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OR4F4/4F5/4F17. Immunogen sequence location: 51 - 100
Reactive Species
A30873 is reactive to OR4F17 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
34.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30873 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ELISA: 1:5000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from MCF-7 cells, using OR4F4/4F5/4F17 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-OR4F4/4F5/4F17 Antibody (A30873)
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Customer Q&As
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4 Customer Q&As for Anti-OR4F4/4F5/4F17 Antibody
Question
We are currently using anti-OR4F4/4F5/4F17 antibody A30873 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
V. Yang
Verified customer
Asked: 2018-09-25
Answer
The anti-OR4F4/4F5/4F17 antibody (A30873) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-25
Question
Will A30873 anti-OR4F4/4F5/4F17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Huang
Verified customer
Asked: 2016-08-04
Answer
It shows on the product datasheet, A30873 anti-OR4F4/4F5/4F17 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-08-04
Question
I was wanting to use your anti-OR4F4/4F5/4F17 antibody for WB for human urinary bladder on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human urinary bladder identification?
V. Anderson
Verified customer
Asked: 2015-11-11
Answer
It shows on the product datasheet, A30873 anti-OR4F4/4F5/4F17 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-11
Question
My question regarding product A30873, anti-OR4F4/4F5/4F17 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Anderson
Verified customer
Asked: 2015-02-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30873 anti-OR4F4/4F5/4F17 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-27
