Click image to see more details
Product Info Summary
| SKU: | A15973 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-OR4N4 Antibody
SKU/Catalog Number
A15973
Size
100ul
Form
Liquid
Description
Boster Bio Anti-OR4N4 Antibody (Catalog# A15973). Tested in WB, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OR4N4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15973)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human OR4N4. Immunogen sequence location: 56 - 105
Reactive Species
A15973 is reactive to OR4N4 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
35.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15973 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells, using OR4N4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-OR4N4 Antibody (A15973)
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Customer Q&As
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4 Customer Q&As for Anti-OR4N4 Antibody
Question
We are currently using anti-OR4N4 antibody A15973 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
The anti-OR4N4 antibody (A15973) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-14
Question
Is a blocking peptide available for product anti-OR4N4 antibody (A15973)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We do provide the blocking peptide for product anti-OR4N4 antibody (A15973). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-16
Question
Would anti-OR4N4 antibody A15973 work on bovine WB with left testis?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
Our lab technicians have not validated anti-OR4N4 antibody A15973 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-OR4N4 antibody A15973 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine left testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-13
Question
Does A15973 anti-OR4N4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Parker
Verified customer
Asked: 2015-06-12
Answer
As indicated on the product datasheet, A15973 anti-OR4N4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-12
